A requirement for antigen-specific helper T cells in the generation of cytotoxic T cells from thymocyte precursors
نویسنده
چکیده
Thymocytes cultured with irradiated, allogeneic stimulator cells yield no cytotoxic effector cells after a period in culture. If, however, a population of irradiated spleen cells syngeneic to the responder cells are added to these cultures, cytotoxicity is generated. The helper activity present in the irradiated syngeneic spleen cells was found to be mediated by a cell bearing theta antigens. Furthermore, it was found to be antigen specific; helper cells which were tolerant of the stimulator cell antigens were unable to help the thymocyte responder cells, although these tolerant cells did contain helpers specific for a third party antigen. These experiments are consistent with a requirement for associative recognition of linked determinants in the induction of killer precursors which is thus strictly analogous to the induction of B-cell precursors via collaboration with helper T cells. In more extensive studies, it was found that histoincompatible helper cells (H-2b, H-2p, H-2q) were able to help a cytotoxic T cell (H-2k) response to a third party stimulator cell antigen (H-2d); that is, the helper T cells which interact with cytotoxic T-cell precursors are not strain specific. It seems likely that the histocompatible helper cells induce killer precursors in an antigen-specific cooperation event similar or identical to normal syngeneic cooperation.
منابع مشابه
In vitro generation of antigen-specific helper T cells that collaborate with cytotoxic T-cell precursors
Antigen-specific helper T cells are required in the generation of cytotoxic T cells from thymocyte precursors. We have demonstrated that these alloantigen-specific helper cells can be generated in vitro and that both the quantity and quality of the helpers appear to be superior to the help obtained from unprimed spleen cells. Optimal helper cell activity is produced at day two of culture when C...
متن کامل1238 A ROLE FOR HELPER T CELLS IN ConA - STIMULATED CYTOTOXICITY
Polyclonal activation by mitogens of precursor cells to yield effector cells is commonly regarded as a result of the direct action of the mitogen on the precursor cell. Such a view predicts that isolated killer or B-cell precursors can be stimulated by mitogens to yield effector cells in the absence of any other cell type. In this series of experiments we show that the polyclonal production of ...
متن کاملAntigen-specific CD4 cells assist CD8 T-effector cells in eliminating keratinocytes.
Keratinocytes expressing tumor or viral antigens can be eliminated by antigen-primed CD8 cytotoxic T cells. CD4 T-helper cells help induction of CD8 cytotoxic T cells from naive precursors and generation of CD8 T-cell memory. In this study, we show, unexpectedly, that CD4 cells are also required to assist primed CD8 effector T cells in rejection of skin expressing human growth hormone, a neo-se...
متن کاملThe role of T helper 9(Th9) against Infectious Diseases
Background and aims: Infectious diseases are disorders caused by organisms such as bacteria, viruses, fungi or parasites .The Th9 subset develops in response to combined signals from TGF-b and IL-4 among a cacophony of other cytokines in an extracellular milieu. T helper 9 (Th9) cells, as a novel CD4 T cell subset, seem to play a complex role in the outcome of specific immune responses. In thi...
متن کامل710 Helper T Cells in the Generation
Although cooperative interactions between helper T cells and B-cell precursors have been extensively characterized (1, 2), the role of helper T cells in the triggering and differentiation of cytotexic T-cell precursors remains ambiguous. Several laboratories have described synergistic interactions between T cells recognizing H-2K or D antigens (SD) and T cells recognizing Ia antigens (LD); incr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Experimental Medicine
دوره 145 شماره
صفحات -
تاریخ انتشار 1977